Te buffer recipe4/16/2023 Using Tris base : To make 1 liter, dissolve 121 g Tris Base in 800 ml of water. Otherwise, sterilize solutions by autoclaving for 20 minutes. If sterile water and sterile stocks are used, there is no need to autoclave. Use concentrated stock solutions to prepare. TE buffer: 10 mM Tris-Cl (pH, usually 7.6 or 8.0) 1 mM EDTA (pH 8.0) Passing the 5x or 10x buffer stocks through a 0.22μm filter can prevent or delay formation of precipitates. However, 5x stock solution is more stable because the solutes do not precipitate during storage. Some investigators prefer to use more concentrated stock solutions of TBE (10x as opposed to 5x). The pH of the concentrated stock buffer should be approx. Prepare a 5x stock solution in 1 liter of H2O: 54 g of Tris base 27.5 g of boric acid 20 ml of 0.5 M EDTA (pH 8.0) Beginning Molecular Biology Laboratory Manualĭepartment of Biological Sciences, 1000 Hilltop Circle Baltimore, MD 21250
0 Comments
Leave a Reply.AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |